By Kevan M. A. Gartland, Michael R. Davey
Agrobacterium Protocols bargains starting and skilled researchers the main complete choice of step by step protocols for the genetic manipulation of crops utilizing Agrobacterium. the subjects diversity from the upkeep of bacterial tradition collections to facets of the metabolism and body structure of reworked tissues and transgenic vegetation. Drawing at the paintings of prime scientists from laboratories all over the world, Agrobacterium Protocols offers a wealth of options for introducing particular DNA sequences into goal plant species and discusses the environmental implications of genetically engineered crops. Its exact systems will facilitate fast move of complex suggestions to different laboratories and their exploitation in primary and utilized plant biology.
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Extra info for Agrobacterium Protocols (Methods in Molecular Biology Vol 44)
These “binary” Ti plasmid vectors were developed based on the finding that the T-DNA region does not have to be physically linked to the vir genes of a Ti plasmid (7). The vir region contains seven operons, of which four (virA, virG, virB, and virD) code for a variety of proteins which are absolutely essential for the transfer process, whereas the remaining three operons (virC, virE, and virF) are necessary only in certain host species (8). The products of virA and virG genes form a twocomponent regulatory system that induces vir gene expression in response to plant wound signals (9).
Tumefaciens is significantly smaller than E. cc&, and has very characteristic motility (4). Behavior and speed measurements are made during slow-motion playback of recorded motility, using a Hitachi (Tokyo, Japan) HV-720K CCTV camera coupled to a Ferguson 3V23 video recorder (Osaka, Japan), a Panasonic WJ-8 10 time-date generator, and a Hitachi VM-920K video monitor. 2. ). When inoculated into the center of such plates, the Chemotaxis Protocols 31 bacteria utilize nutrients, thus creating a concentration gradient.
A , and Theologis, A. (199 1) Reversible inhibition of tomato fruit senescence by antisense RNA. Science 254, 437439. 25. Teeri, T. , and Palva, E. T. (1986) Identification of plant promoters in situ by T-DNA-medlated transcriptional fusion to the npt-II gene. EMBO J. 5, 1755-1760. 26. Fobert, P. , Miki, B. L , and Iyer, V. N. (1991) Detection of gene regulatory signals m plants revealed by T-DNA-mediated fusions. Plant Mol. Biol 17,837-852. 27 Gierl, A. and Saedler, H. (1992) Plant-transposable elements and gene taggmg.
Agrobacterium Protocols (Methods in Molecular Biology Vol 44) by Kevan M. A. Gartland, Michael R. Davey