J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D.'s Advanced Techniques in Biological Electron Microscopy III PDF

By J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)

ISBN-10: 3540164006

ISBN-13: 9783540164005

ISBN-10: 3642711359

ISBN-13: 9783642711350

This quantity is a continuation of 2 previous books on complicated electron microscope options. the aim of this sequence has been to supply in­ intensity analyses of equipment that are thought of to be on the innovative of electron microscopic learn approaches with functions within the organic sciences. The challenge of the current quantity is still that of a resource ebook for the examine practitioner or complex scholar, specifically one already good versed in uncomplicated electron optical equipment. it's not intended to supply in­ troductory fabric, nor can this modest quantity desire to hide the whole spectrum of complicated know-how now on hand in electron microscopy. long ago decade, desktops have discovered their method into many study laboratories due to the big raise in computing energy and stor­ age to be had at a modest fee. The ultrastructural sector has additionally benefited from this growth in a couple of methods with a view to be illustrated during this quantity. 1/2 the contributions talk about applied sciences that both at once or in a roundabout way make broad use of desktop methods.

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SIRT uses a linear error definition similar to ART (7), but the error is now calculated for all projections simultaneously and the corrections are applied to each voxel of the volume such that the average error for each voxel is minimized. ILST uses as an error measure the squared difference between each original and recalculated projection weighted by the inverse variance along each projection ray. This error is minimized by applying the corrections for each projection direction, one at a time.

1983, 1984). A detailed comparison of these results with those obtained by KNAUER et al. is made difficult by the large discrepancy in the resolutions used for display. However, as stated at the beginning of this section, all three models appear to agree on the level of gross morphology, in terms of the appearance of the platform and the overall shape of the particle (Fig. 25 a). VAN HEEL (1983 b) and VERSCHOOR et al. , averages formed over defined subsets; see FRANK 1982 b) of computer-classified particle views.

The lower panels show the same sections; a 2 X enlargement of the subunit with superimposed contours Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies 27 In order to give a representation that reflects the uncertainty about structural details below this level, the reconstructed density array (which represents a single slice of the entire three-dimensional model, see Fig. 9) must be spatially filtered to that resolution (Fig. 11). , a resolution that is independent of the location in the object).

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Advanced Techniques in Biological Electron Microscopy III by J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)

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